<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Maïwenn Kersaudy-Kerhoas</style></author><author><style face="normal" font="default" size="100%">Deirdre M. Kavanagh</style></author><author><style face="normal" font="default" size="100%">Resham S. Dhariwal,</style></author><author><style face="normal" font="default" size="100%">Colin J. Campbell</style></author><author><style face="normal" font="default" size="100%">Desmulliez, M.P.Y.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Validation of a blood plasma separation system by biomarker detection</style></title></titles><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><publisher><style face="normal" font="default" size="100%">Lab-on-chip</style></publisher><abstract><style face="normal" font="default" size="100%">A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood. The microfluidic network features a series of constrictions and bifurcations to enhance the product yield and purity. A maximum purity efficiency of 100% is obtained on blood with entrance hematocrit level up to 30% with a flow rate of 2 mL h−1. Flow cytometry was performed on the extracted plasma to evaluate the separation efficiency and to assess cell damage. A core target of this study was the detection ofcell-free DNA from the on-chip extracted plasma. To this effect, PCR was successfully carried out off-chip on the cell-free DNA present in the plasma extracted on-chip. A house-keeping gene sequence (GAPDH) was amplified without the need for a purification after the separation, thereby showing the high quality of the plasma sample. The resulting data suggests that the system can be used as a preliminary module of a total analysis system for cell-free DNA detection in human plasma.
</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue></record></records></xml>
